The FLIPR assay was carried out on FDSS/µCell with ligand addition at 10?s for your binding assay and mAb1/isotype addition at ten?s followed by hCCL1 addition at 300?s to the Opposition binding assay, with a complete checking of 500?s. The excitation and emission wavelengths were being established at 485?nm and 525?nm, respectively. Once the run,